Singapore-MIT Alliance for Research & Technology

Biosystems and Micromechanics

1, CREATE WAY
#04-13/14 Enterprise Wing and #B-101
Singapore 138602

BioSystems and Micromechanics (BioSyM) - IRG

Thorsten Wohland
Associate Professor, Chemistry Department, National University of Singapore (NUS)

http://staff.science.nus.edu.sg/~chmwt/

Positions

  • 2008- Associate Professor, Chemistry Department, National University of Singapore.
  • 2002- Assistant Professor, Chemistry Department, National University of Singapore.
  • 2000-2002 Postdoctoral Fellow, Department of Chemistry, Stanford University, USA.
  • 1997-2000 Department of Chemistry, Ecole Federal Polytechnique de Lausanne (EPFL, Swiss Federal Institute of Technology), Switzerland, Dr. es sc. in Biophysics (equiv. PhD)
  • 1996-1997 Research Scientist, Institute of Inhalation Biology, GSF-Research Center for Environment and Health, Neuherberg, Germany (performed during national service)
  • 1994-1995 European Molecular Biology Laboratory (EMBL) Heidelberg, Diploma thesis in Physics
  • 1992-1995 Department of Physics and Astronomy, University of Heidelberg, Diploma in Physics
  • 1989-1991 Professor Department of Physics, Technical University of Darmstadt, Pre-Diploma in Physics
  • 1996-1997 Research Scientist, Institute of Inhalation Biology, GSF-Research Center for Environment and Health, Neuherberg, Germany (performed during national service)

Selected peer-reviewed publications

  • P. Liu., T. Sudhaharan, R.M.L. Koh, L.C. Hwang, S. Ahmed, I.N. Maruyama, and T. Wohland. Investigation of the dimerization of proteins from the epidermal growth factor receptor family by single wavelength fluorescence cross-correlation spectroscopy. Biophys. J. (93): 684-698 (2007).
  • B. Kannan, G. Lin , S. Thankiah, S. Ahmed, I.N. Maruyama, and T. Wohland. Spatially Resolved Total Internal Reflection Fluorescence Correlation Microscopy Using an Electron Multiplying Charge-Coupled Device Camera. Anal. Chem., (79) 4463-4470 (2007).
  • P.K. Ang, K.P. Loh, T.Wohland, M. Nesladek, E. Van Hove. Supported Lipid Bilayer on Nanocrystalline Diamond: Dual optical and field effect Sensor for Membrane Disruption, Adv. Func. Mat. 19(1):109-116 (2009).
  • S. Leptihn, J.Y.Har, J. Chen, B. Ho, T. Wohland, J.L. Ding. Single molecule resolution of the antimicrobial action of quantum dot-labeled sushi peptide on live bacteria, BMC Biology 2009, 7:22.
  • T. Sudhaharan, P. Liu, Y.H. Foo1, W. Bu1, K.B. Lim, T. Wohland, S. Ahmed, Determination of in vivo dissociation constants, KD, of Cdc42-effector complexes in living mammalian cells using single wavelength fluorescence cross-correlation spectroscopy (SW-FCCS), JBC 284: 13602-13609 (2009).
  • D. Zhang, M. Manna, T. Wohland, and R.S. Kraut. Alternate raft pathways cooperate to mediate slow diffusion and efficient uptake of a sphingolipid tracer to degradative vs. Recycling pathways, JCS, 2009; 122(20): 3715-3728.
  • X. Shi, Y.H. Foo, T. Sudhaharan, S.-W. Chong, V. Korzh, S. Ahmed, T. Wohland, Determination of dissociation constants in living zebrafish embryos with single wavelength fluorescence cross-correlation spectroscopy, Biophys. J. (97)2:678-686 (2009).

Current research support

  • SBIC: Development of New Fluorescent Probes and Labelling Techniques for in-Vivo Multi-Wavelength and Time-Resolved Spectroscopy of Biomolecules: Investigation of Cytoskeleton and Lipid Microdomain Dynamics (003/2005) 2005-2008.
  • NUS-ARF: Multicolor spectroscopy for the detection and investigation of photosensitizer action in cancer tissues, 2007-2010.
  • BMRC/NMRC: Mechanistic studies of Epidermal Growth Factor Receptor and Cdc42 signalling in vivo using fluorescence spectroscopy techniques, 2008-2011.
  • ARC(MOE): An integrated approach to image guided spectroscopy for the evaluation of biomolecular interactions, 2008-2011.